Like many cultivars in the cannabis universe, the origin of Dark Knight varies depending on where you look. Two of the possible sources include botanist Daniel Hernadez and KDS Seeds. Like the origin, Dark Knight strains vary in the genetic lineage with each breeder using different parents to create their rendition of the Caped Crusader.

Most online sources say Dark Knight is a cross between Grand Daddy Purple and Purple Kush. Others — such as Generation Health — claim the cultivar is Blueberry and Kryptonite, while the seed bank Seed City believes it to be Black Widow x Mendo Purps crossed with Trinity x JSD.